LOCUS       sequence-110915-       15606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon-optimized human dynein-2 motor domain (D1091–Q4307) for 
            baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to 
            cleave 6His-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Schmidt H, Zalyte R, Urnavicius L, Carter AP
  TITLE     Structure of human cytoplasmic dynein-2 primed for its power stroke.
  JOURNAL   Nature. 2015 Feb 19;518(7539):435-8. doi: 10.1038/nature14023. Epub 
            2014 Dec 1.
  PUBMED    25470043
REFERENCE   2  (bases 1 to 15606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14023"; journalName: "Nature"; date: "2015-02-19- 
            19"; volume: "518"; issue: "7539"; pages: "435-8"
COMMENT     Sequence Label: pFastBac-GFP-dynein-2(D1091–Q4307)
FEATURES             Location/Qualifiers
     source          1..15606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(589..657,658..1449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4062..4085
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             4128..4301
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             4302..4475
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             4533..4553
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4575..4595
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    15096..15230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15133..15152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15187..15206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15259..15424
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacc
     4081 atcacggttc tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg gacaacaaat
     4141 tcaacaaaga acaacaaaac gcgtactatg agatcttaca tttacctaac ttaaacgaag
     4201 gacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt
     4261 tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca
     4321 aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac
     4381 gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag
     4441 aagctgaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc gcggggaagt
     4501 caaccgatta tgatattcca actactgcta gcgagaacct atatttccag ggagattatg
     4561 atatccctac gactgagaat ctttattttc agggcatgtc taaaggtgaa gaattattca
     4621 ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg
     4681 tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta
     4741 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcggt tatggtgttc
     4801 aatgttttgc gagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc
     4861 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca
     4921 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg
     4981 attttaaaga agatggtaac attttaggtc acaaattgga atacaactat aactctcaca
     5041 atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc aaaattagac
     5101 acaacattga agacggttct gttcaattag ctgaccatta tcaacaaaat actccaattg
     5161 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca
     5221 aggatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta
     5281 ttaccggttc tgacgatttg gaagtgacac gcaagaagct ggtcgatgac tgtcaccact
     5341 tccgcctcga agaacctaac ttctctctcg cctccagcat cagcaaggat attgagtcct
     5401 gtgctcagat ttgggccttc tacgaagagt tccagcaggg attccaagaa atggccaatg
     5461 aagattggat cacctttcgc actaagacct acttgtttga ggagtttctg atgaattggc
     5521 acgaccgcct caggaaagtg gaggagcatt ctgtgatgac tgtcaagctc caatctgagg
     5581 tggacaaata taagattgtt atccctatcc tgaagtacgt ccgcggagaa cacctgtcac
     5641 ccgatcactg gctggatctg ttccgcttgc tgggtctgcc tcgcggcaca tctctggaga
     5701 aactgctgtt cggtgacctg ctgagagttg ccgataccat cgtggccaag gctgctgacc
     5761 tgaaagatct gaactcacgc gcccagggtg aagtgaccat ccgcgaagca ctcagggaac
     5821 tggatttgtg gggcgtgggt gctgtgttca cactgatcga ctatgaggac tcccagagcc
     5881 gcaccatgaa gctgatcaag gattggaagg acatcgtcaa ccaggtgggc gacaatagat
     5941 gcctcctgca gtccttgaag gactcaccat actataaagg ctttgaagac aaggtcagca
     6001 tctgggaaag gaaactcgcc gaactggacg aatatttgca gaacctcaac catattcaga
     6061 gaaagtgggt ttacctcgaa ccaatctttg gtcgcggagc cctgcccaaa gagcagacca
     6121 gattcaacag ggtggatgaa gatttccgca gcatcatgac agatatcaag aaggacaatc
     6181 gcgtcacaac cttgactacc cacgcaggca ttcgcaactc actgctgacc atcctggacc
     6241 aattgcagag atgccagcgc agcctcaacg agttcctgga ggagaagcgc agcgccttcc
     6301 ctcgcttcta cttcatcgga gacgatgacc tgctggagat cttgggccag tcaaccaatc
     6361 catccgtgat tcagtctcac ctcaagaagc tgtttgctgg tatcaactct gtctgtttcg
     6421 atgagaagtc taagcacatt actgcaatga agtccttgga gggagaagtt gtgccattca
     6481 agaataaggt gcccttgtcc aataacgtcg aaacctggct gaacgatctg gccctggaga
     6541 tgaagaagac cctggagcag ctgctgaagg agtgcgtgac aaccggacgc agctctcagg
     6601 gagctgtgga cccttctctg ttcccatcac agatcctgtg cttggccgaa cagatcaagt
     6661 ttaccgaaga tgtggagaac gcaattaaag atcactccct gcaccagatt gagacacagc
     6721 tggtgaacaa attggagcag tatactaaca tcgacacatc ttccgaggac ccaggtaaca
     6781 cagagtccgg tattctggag ctgaaactga aagcactgat tctcgacatt atccataaca
     6841 tcgacgtggt caagcagctg aaccaaatcc aagtgcacac caccgaagat tgggcctgga
     6901 agaagcagtt gaggttctac atgaagtccg accacacctg ttgcgttcag atggttgaca
     6961 gcgagttcca gtacacctat gagtaccaag gaaatgccag caagctcgtt tacactccac
     7021 tcactgacaa gtgttacctc accttgacac aggctatgaa gatgggcctg ggaggcaacc
     7081 catacggtcc agctggcact ggtaagacag agagcgttaa ggcactcgga ggtctgctgg
     7141 gcaggcaggt cctcgtgttc aactgtgatg aaggaatcga cgttaagtcc atgggaagaa
     7201 tctttgttgg cctcgttaag tgtggagctt ggggttgctt cgacgagttc aacaggctgg
     7261 aggaatctgt gctgagcgcc gtctctatgc agatccagac catccaggac gcattgaaga
     7321 accacaggac cgtctgcgag ctgttgggta aggaagtgga ggtgaactcc aactccggaa
     7381 tcttcatcac aatgaatccc gcaggtaaag gatatggagg aagacagaaa ctcccagaca
     7441 acctgaagca gctgttccgc ccagtggcta tgtcccatcc agacaatgag ctgatcgccg
     7501 aagtcatcct ctattccgag ggattcaaag atgctaaagt tctctccaga aagctcgtgg
     7561 ccatcttcaa tctgtcaaga gaactcctga cacctcagca gcattacgac tggggtctga
     7621 gagccctcaa gaccgtcctg agaggttcag gaaatctcct caggcagctg aacaagagcg
     7681 gtacaacaca gaatgcaaat gagagccaca ttgtcgtcca ggctctgagg ctgaatacca
     7741 tgtcaaagtt cacattcaca gactgcacaa gatttgacgc tctgattaaa gatgtgttcc
     7801 ctggtattga actcaaagaa gtggagtatg acgagctgag cgccgctttg aagcaggtgt
     7861 ttgaggaggc taactatgag attatcccta atcagatcaa gaaagcattg gaactgtatg
     7921 aacagctgtg tcagaggatg ggagtggtga ttgtgggccc atcaggcgca ggtaagagca
     7981 ctctctggag aatgctgaga gcagcactgt gcaagactgg aaaggtggtg aagcaataca
     8041 ccatgaatcc caaggccatg cccaggtacc aactgctggg ccatatcgac atggacacca
     8101 gagaatggag cgacggcgtg ctcacaaact ccgccagaca agtcgtgcgc gaacctcaag
     8161 acgtcagctc ttggatcatc tgcgatggtg atattgaccc tgagtggatc gagtccctga
     8221 attccgtgtt ggatgacaac aggctcctca caatgccttc tggtgagaga atccagttcg
     8281 gtcctaacgt gaacttcgtg ttcgagacac acgatctcag ctgtgctagc ccagctacta
     8341 tctcccgcat gggaatgatc ttcttgtccg acgaggagac agatttgaac tcattgatca
     8401 agtcttggct cagaaaccag cctgcagaat ataggaataa cctggagaac tggatcggtg
     8461 attacttcga gaaggctttg cagtgggtgc tgaaacagaa cgactatgtc gtcgaaacca
     8521 gcctggtcgg tacagttatg aacggactct cccatctgca cggatgcaga gatcacgatg
     8581 agtttatcat caatttgatc cgcggactgg gaggtaactt gaatatgaaa tctcgcctgg
     8641 agttcactaa agaagtgttc cactgggcta gggagtcacc acctgacttc cacaaaccta
     8701 tggacaccta ctatgattcc acaagaggca ggttggccac ctacgtgctg aagaagcctg
     8761 aggacctcac cgctgacgac ttctccaacg gactgactct gcccgtgatc cagactccag
     8821 acatgcagcg cggactcgat tactttaagc cctggctcag ctccgatacc aagcaacctt
     8881 tcattctcgt gggaccagag ggatgtggta aaggaatgct cctgaggtat gcattctccc
     8941 agctccgctc aacccaaatt gccactgttc actgttcagc ccaaacaact tcaaggcatc
     9001 tcctccagaa gctcagccag acctgtatgg ttatcagcac caacaccggc agagtttacc
     9061 gcccaaagga ttgtgagcgc ctcgtgttgt acctcaaaga tatcaatctg cccaaactcg
     9121 ataagtgggg cacttccacc ctcgtggcat ttctccaaca ggtgctgacc taccagggct
     9181 tctacgacga gaacctggag tgggtcggat tggagaacat ccagattgtg gcttccatgt
     9241 ctgccggcgg taggttggga aggcataagc tcaccaccag gtttacatca attgtgagac
     9301 tgtgctcaat cgattatccc gagcgcgaac agttgcagac catctatggc gcctacctcg
     9361 aacctgtcct ccacaagaat ttgaagaacc atagcatctg gggctcatca agcaagatct
     9421 acctcttggc tggctctatg gttcaggtgt acgaacaagt ccgcgccaag ttcaccgtcg
     9481 atgattactc acattacttc ttcacaccct gcattctgac acaatgggtt ctgggactgt
     9541 ttcgctatga cctggaggga ggctcatcaa accacccatt ggattatgtc ctcgaaatcg
     9601 tggcttacga agcccgcagg ctctttagag ataagattgt tggcgctaag gaactgcatc
     9661 tgttcgatat tatcctcacc tctgtgtttc aaggtgattg gggctctgat atcctggata
     9721 atatgtccga ttccttctat gtgacatggg gagccaggca caactccggt gcaagggctg
     9781 ctccaggcca accattgcca cctcatggca agcctctcgg caaactgaac tcaactgact
     9841 tgaaggacgt gatcaagaag ggcctgattc actacggccg cgacaaccag aacctggaca
     9901 ttctgttgtt ccacgaggtc ctggagtata tgtccagaat tgatcgcgtc ctgtctttcc
     9961 ctggtggatc actgctgctg gccggacgct ctggagttgg aagacgcact attacttctc
    10021 tggtcagcca tatgcatgga gccgtcctgt tctctccaaa gatcagcaga ggctacgaac
    10081 tcaagcaatt caagaacgat ctgaaacacg tcttgcaact cgccggtatc gaggcccagc
    10141 aggtcgtcct cctcttggag gactatcaat tcgtccaccc aaccttcctg gagatgatca
    10201 actccctgct gtcctctggc gaggtgcccg gcttgtacac tttggaggaa ctggagccac
    10261 tgctcctccc attgaaggat caggcatcac aggacggctt cttcggccca gtgttcaatt
    10321 acttcaccta tcgcattcaa cagaatctcc acattgtgct gattatggac agcgctaatt
    10381 ccaatttcat gatcaattgc gagagcaatc ccgccctcca taagaagtgc caggtcctct
    10441 ggatggaggg ttggtctaat tcttctatga agaagattcc cgagatgttg tttagcgaga
    10501 ctggaggcgg tgagaagtac aacgacaaga agcgcaaaga ggagaagaag aagaactccg
    10561 tcgatcctga tttcctcaag agcttcctgc tgatccacga gtcttgcaaa gcttacggag
    10621 ctactcctag ccagtacatg accttcctcc acgtctattc cgccatctcc agctcaaaga
    10681 agaaggagct gctgaagcgc caatctcatc tgcaggccgg agtcagcaag ctgaacgaag
    10741 ccaaagctct ggtggatgaa ctgaatcgca aggctggcga acaatcagtc ctcctgaaga
    10801 ctaagcagga tgaagctgac gctgccctgc aaatgattac cgtgtctatg caggatgctt
    10861 ccgagcagaa gacagagctg gagaggctga agcaccgcat cgcagaggag gtggtcaaga
    10921 tcgaggagag aaagaacaag attgacgacg aactcaaaga ggtgcagcct ctggtgaacg
    10981 aggccaagct cgccgtgggt aatatcaaac cagagtctct ctcagagatc aggtcactga
    11041 gaatgccacc agacgttatc cgcgacatcc tggagggcgt cctgcgcttg atgggtatct
    11101 ttgacacctc ttgggtgtct atgaagtctt tcttggccaa gcgcggtgtc agggaggaca
    11161 ttgctacttt cgacgctagg aacatctcca aggaaattag ggaatctgtg gaggaactgc
    11221 tgttcaagaa taaaggttca tttgatccca agaacgctaa gagagcatca actgcagctg
    11281 cacccttggc agcctgggtt aaggccaata tccagtactc tcacgtgctc gaacgcatcc
    11341 accctctgga gactgagcaa gccggcctgg aaagcaacct gaagaagacc gaggatagaa
    11401 agaggaaact ggaagaactc ctcaattctg tcggtcagaa ggtgtcagaa ctgaaggaga
    11461 aatttcagag caggacctca gaagcagcta agttggaagc tgaggtgtcc aaggctcagg
    11521 agactatcaa agcagctgaa gtgttgatta atcagctgga ccgcgaacac aagagatgga
    11581 atgctcaggt cgtcgagatt actgaggagc tggcaaccct cccaaagagg gctcagttgg
    11641 ccgcagcctt tatcacctac ttgtccgctg cacctgagtc tctcagaaag acatgtctgg
    11701 aggagtggac caagtctgcc ggcctggaga agtttgacct gagaagattt ctctgcaccg
    11761 agtcagagca gctgatctgg aagtctgaag gtctgcccag cgatgacctc tcaatcgaga
    11821 acgcactggt tatcttgcaa tcccgcgttt gccctttcct catcgatccc agctcacagg
    11881 ctactgagtg gctgaagact cacttgaagg attccaggct ggaagtgatc aaccagcaag
    11941 actccaactt catcactgcc ctggaactcg ccgtccgctt cggcaagacc ttgatcatcc
    12001 aggagatgga tggcgtggag ccagttctgt accctctgtt gcgcagagat ttggtggccc
    12061 aaggcccacg ctatgtggtc cagattggag ataagatcat cgactataac gaggagtttc
    12121 gcctgttcct gagcactagg aatcccaacc cattcattcc accagacgct gccagcatcg
    12181 tcacagaggt taatttcaca accacccgct caggactgag aggccagctc ctggccctca
    12241 ccatccaaca tgagaaaccc gatttggaag aacagaagac taagctgctc caacaggagg
    12301 aggataagaa gatccaactg gccaaactcg aagaatctct gctggagaca ttggctactt
    12361 ctcagggcaa catcctggag aacaaggacc tgatcgagtc tctgaatcag actaaagcat
    12421 cttccgccct catccaagag tctctgaagg aatcttataa gctgcaaatc tctctcgacc
    12481 aggagcgcga cgcatatctg ccactcgctg agtctgcttc aaagatgtac ttcattatct
    12541 ctgacctctc caagatcaat aatatgtaca ggttctccct ggccgccttt ctgaggttgt
    12601 ttcaaagggc actccagaat aagcaggatt ctgagaacac agaacagagg attcaatccc
    12661 tgatcagctc cctgcagcac atggtgtacg aatacatctg ccgctgcctg ttcaaggccg
    12721 accagctgat gttcgccctg catttcgtca gaggtatgca tccagaattg ttccaggaga
    12781 acgaatggga taccttcact ggcgtggtgg tcggagacat gttgcgcaaa gccgactccc
    12841 agcagaagat cagggaccag ttgccttcat ggatcgatca agagagatcc tgggctgtgg
    12901 caaccttgaa gatcgctctg ccttccctgt accagactct gtgtttcgag gacgccgctt
    12961 tgtggcgcac ctactacaac aacagcatgt gtgagcagga atttcctagc atcttggcta
    13021 agaaggtcag cttgttccag cagatcctcg tcgtgcaggt gctcaggcca gatagattgc
    13081 agtcagctat ggccctcttt gcctgcaaga ccttgggttt gaaggaagtc tctccactcc
    13141 cactcaatct caagaggctg tacaaggaga cactcgaaat cgagcccatt ctgatcatta
    13201 tttctccagg agccgatccc tcccaggaac tccaggagct cgccaacgcc gaaagatcag
    13261 gagaatgtta ccaccaggtt gcaatgggcc agggccaagc tgacttggct atccaaatgc
    13321 tcaaggaatg cgcaaggaat ggtgactggc tgtgtttgaa gaacttgcac ctggttgtct
    13381 cctggctgcc tgtcttggag aaggaactga acaccttgca acccaaagac actttcaggc
    13441 tctggttgac agccgaagtg cacccaaact ttacacctat cctcctccag tcaagcctca
    13501 agatcactta tgaatcacca cctggactca agaagaatct catgcgcaca tatgaatctt
    13561 ggacacctga acaaatctct aagaaagata acactcaccg cgcacatgct ctgttctctc
    13621 tggcctggtt tcacgccgca tgccaagaga ggcgcaacta cattcctcaa ggttggacca
    13681 aattctacga gttctctctc tccgacctga gggccggtta caatatcatt gacaggctct
    13741 ttgatggtgc caaggacgtg caatgggagt ttgtccatgg tctgctggag aacgccatct
    13801 acggaggccg catcgataac tatttcgact tgcgcgtcct gcagagctat ttgaagcaat
    13861 tcttcaactc tagcgtcatt gacgtgttca atcagcgcaa caagaagtcc atctttccct
    13921 acagcgtgag cctgccacaa agctgcagca ttctggatta cagggctgtc attgagaaga
    13981 ttccagaaga cgataagcca tccttcttcg gtctgcctgc caacattgca cgctcatcac
    14041 agcgcatgat ctcatctcag gtgatttccc aactccgcat cctgggccgc tctattactg
    14101 caggctcaaa gttcgatcgc gagatctgga gcaatgaact cagcccagtg ctcaacctgt
    14161 ggaagaaact gaaccagaac tccaacctga tccaccagaa ggtccctcct cccaatgacc
    14221 gccaaggatc accaattctg tcctttatta tcttggagca gttcaacgcc atcagattgg
    14281 tccagtcagt tcatcaatca ctggcagcct tgagcaaagt gatccgcggc actacactgc
    14341 tctcatcaga agtccagaag ttggcctctg ccctgctcaa ccagaagtgc cctctggcct
    14401 ggcaatccaa gtgggaggga cccgaagacc ctctgcaata tctcagaggc ctcgtggcta
    14461 gagcactggc catccagaat tgggtcgata aagcagagaa gcaggccctc ttgtccgaaa
    14521 cactggacct ctctgaattg ttccatcccg acacattcct gaacgccctc cgccaggaaa
    14581 cagcaagggc tgttggaaga tcagtggatt ctctgaaatt tgtcgcctcc tggaagggta
    14641 gactgcaaga ggccaaactc cagatcaaga tctcaggact gctcctggaa ggctgctcct
    14701 tcgacggtaa tcaactgtcc gagaatcagc tcgacagccc aagcgtctct agcgttctgc
    14761 catgcttcat gggttggatt cctcaagacg cttgcggccc atactcaccc gatgagtgca
    14821 tttctttgcc agtgtacaca tccgctgagc gcgacagagt cgtgaccaac atcgatgtgc
    14881 cttgcggtgg taatcaggat caatggattc agtgtggagc cgccttgttt ctcaagaatc
    14941 aggtctaact cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca
    15001 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    15061 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    15121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    15181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactga
    15241 tatcgcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
    15301 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
    15361 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
    15421 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
    15481 gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc
    15541 tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc
    15601 gaatgg
//
