LOCUS       sequence-110920-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression cassette carried by a yeast single-copy episome 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: Cas9-NAT
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..37)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actcgagaag agatcacacc ttcctcttct tcttggggtc agccctgctg tctccaccga
       61 gctgagagag gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg
      121 cgtccaggac ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact
      181 tgaaggctgc aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct
      241 gctccctgat gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag
      301 cgtcggcgag gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt
      361 agtgtttgtg ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga
      421 gcttttcata gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct
      481 cgttaccttt ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct
      541 caaagagaga gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt
      601 tcgcctcgag aaagtcgatg gggttttttt cgaagcttga tcgctccatg attgtgatgc
      661 ccagcagttc cttgacgctt ttgagttttt tagacttccc tttctccact ttggccacaa
      721 ccagtacact gtaagcgact gtaggagaat cgaatccgcc gtatttcttg gggtcccaat
      781 cttttttgcg tgcgatcagc ttgtcgctgt tccttttcgg gaggatactt tccttggaga
      841 agcctccggt ctgtacttcg g
//
