LOCUS       sequence-110923-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression cassette carried by a yeast single-copy episome 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: Cas9-NAT
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa
       61 gttttctaga actagtggat cccccgggaa aaatggacaa gaagtactcc attgggctcg
      121 atatcggcac aaacagcgtc ggttgggccg tcattacgga cgagtacaag gtgccgagca
      181 aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg
      241 ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc
      301 gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga
      361 tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg
      421 ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg
      481 aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact gataaggctg
      541 acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca
      601 tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc
      661 agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag
      721 caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc
      781 ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc
      841 ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca
      901 cc
//
