LOCUS       sequence-110924-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression cassette carried by a yeast single-copy episome 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: Cas9-NAT
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(110..121)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gccttagtca gattatcgaa cttccgttgt gtgatcagtt tggcgttcag cagctgccgc
       61 caataatttt tcattttctt gacaacttct tctgagggga cgttatcact cttccctcta
      121 tttttatcgg atcttgtcaa cactttatta tcaatagaat catctttgag aaaagactgg
      181 ggcacgatat gatccacgtc gtagtcggag agccgattga tgtccagttc ctgatccacg
      241 tacatgtccc tgccgttctg caggtagtac aggtagagct tctcattctg aagctgggtg
      301 ttttcaactg ggtgttcctt aaggatttgg gaccccagtt cttttatacc ctcttcaatc
      361 ctcttcatcc tttccctact gttcttctgt cccttctggg tagtttggtt ctctcgggcc
      421 atctcgataa cgatattctc gggcttatgc cttcccatta ctttgacgag ttcatccacg
      481 accttaacgg tctgcagtat tccctttttg atagctgggc tacctgcaag attagcgatg
      541 tgctcgtgaa gactgtcccc ctggccagaa acttgtgctt tctggatgtc ctccttaaag
      601 gtgagagagt catcatggat caactgcatg aagttccggt tggcaaatcc atcggactta
      661 agaaaatcca ggattgtctt tccactctgc ttgtctcgga tcccattgat cagttttctt
      721 gacagccgcc cccatcctgt atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg
      781 aagagatgag cgtaagtttt caagcgttct tcaatcatct ccctatcttc aaacaacgta
      841 agggtgagga caatgtcctc aagaatgtcc tcgttctcct cattgtccag gaagtccttg
      901 tctttaatga ttttcaggag atcgtgatac gttcccaggg atgcgttgaa gcga
//
