LOCUS       sequence-110926-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression cassette carried by a yeast single-copy episome 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: Cas9-NAT
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..40)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    81..584
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        621..725
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
       61 taataatggt ttcttagacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta
      121 atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga
      181 ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa
      241 aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag
      301 cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc
      361 gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga
      421 agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga
      481 aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt
      541 tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg
      601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      901 gccccgaaga a
//
