LOCUS       sequence-110927-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression cassette carried by a yeast single-copy episome 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: Cas9-NAT
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        179..522
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
       61 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
      121 tgtcacctta cgtagatctg tttagcttgc cttgtccccg ccgggtcacc cggccagcga
      181 catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg
      241 tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg catccataca
      301 ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca
      361 gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa
      421 tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt
      481 gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtac cactcttgac
      541 gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat cgaggcactg
      601 gatgggtcct tcaccaccga caccgtcttc cgcgtcaccg ccaccgggga cggcttcacc
      661 ctgcgggagg tgccggtgga cccgcccctg accaaggtgt tccccgacga cgaatcggac
      721 gacgaatcgg acgacgggga ggacggcgac ccggactccc ggacgttcgt cgcgtacggg
      781 gacgacggcg acctggcggg cttcgtggtc gtctcgtact ccggctggaa ccgccggctg
      841 accgtcgagg acatcgaggt cgccccggag caccgggggc acggggtcgg g
//
