LOCUS       sequence-110931-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  trp1 deletion gRNA cassette carried by pRS42H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: gRNA-trp-HYB
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..40)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    413..460
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
       61 taataatggt ttcttagtat gatccaatat caaaggaaat gatagcattg aaggatgaga
      121 ctaatccaat tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca
      181 tacgataccc cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct
      241 acataaatag acgcatataa gtacgcattt aagcataaac acgcactatg ccgttcttct
      301 catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta
      361 tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc
      421 tattccgaag ttcctattct ctagaaagta taggaacttc agagcgcttt tgaaaaccaa
      481 aagcgctctg aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact
      541 aaaatattgc gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc
      601 tctgtgctat atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta
      661 aactcgacct ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg
      721 tagtaagaac tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata
      781 cgtagtatat agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc
      841 atcaacgcta tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa
      901 tcggggatgc ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc
      961 gggaa
//
