LOCUS       sequence-110933-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QT6
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             567..584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(637..655)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggctttgacc cggagaaagc tcagtgttgc ctagtggaga atggacagat tttaactcac
      121 ggcagtggag ggaaaggata tggattggca tctacaggag taacttctgg gtgctatcag
      181 tggaagtttt atattgtgaa ggaaaacaga ggtaatgaag gcacgtgtgt tggagtttct
      241 cgctggccag tacatgactt taatcaccgc actacctcgg atatgtggct ctatagggcc
      301 tacagtggta acctctatca caatggagaa cagactctca cattgtccag ctttactcaa
      361 ggagatttca ttacctgtgt gttagacatg gaagccagga ccatttcttt tgggaaaaat
      421 ggagaggaac ccaaattagc ttttgaagat gtggatgcag cagagttgta cccatgtgtg
      481 atgttctata gtagcaatcc aggggaaaag gtgaaaattt gtgatatgca gatgcgttga
      541 tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      601 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataa
//
