LOCUS       sequence-110934-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RCI
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             690..707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcagcgtcg aatcccaccc cgtccttgaa aaactgaagg ctgctcacag ctacaacccg
      121 aaagagtttg agtggaatct gaaaagcggg cgtgtgttca tcatcaagag ctactctgag
      181 gacgacatcc accgctccat taagtactcc atctggtgta gcacagagca cggcaacaag
      241 cgcctggaca gcgccttccg ctgcatgagc agcaaggggc ccgtctacct gctcttcagc
      301 gtcaatggga gtgggcattt ttgtggggtg gccgagatga agtcccccgt ggactacggc
      361 accagtgccg gggtctggtc tcaggacaag tggaagggga agtttgatgt ccagtggatt
      421 tttgttaagg atgtacccaa taaccagctc cggcacatca ggctggagaa taacgacaac
      481 aaaccggtca caaactcccg ggacacccag gaggtgccct tagaaaaagc caagcaagtg
      541 ctgaaaatta tcagttccta caagcacaca acctccatct tcgacgactt tgctcactac
      601 gagaagcgcc aggaggagga ggaggtggtg cgcaaggaac ggcagagtcg aaacaaacaa
      661 tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      721 ctaac
//
