LOCUS       sequence-110935-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RCJ
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             663..680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(733..751)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggccaccccg tccttgaaaa actgaaggct gctcacagct acaacccgaa agagtttgag
      121 tggaatctga aaagcgggcg tgtgttcatc atcaagagct actctgagga cgacatccac
      181 cgctccatta agtactccat ctggtgtagc acagagcacg gcaacaagcg cctggacagc
      241 gccttccgct gcatgagcag caaggggccc gtctacctgc tcttcagcgt caatgggagt
      301 gggcattttt gtggggtggc cgagatgaag tcccccgtgg actacggcac cagtgccggg
      361 gtctggtctc aggacaagtg gaaggggaag tttgatgtcc agtggatttt tgttaaggat
      421 gtacccaata accagctccg gcacatcagg ctggagaata acgacaacaa accggtcaca
      481 aactcccggg acacccagga ggtgccctta gaaaaagcca agcaagtgct gaaaattatc
      541 agttcctaca agcacacaac ctccatcttc gacgactttg ctcactacga gaagcgccag
      601 gaggaggagg aggtggtgcg caaggaacgg cagtgatgac gaagcttgcg gccgcactcg
      661 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      721 tggctgctgc caccgctgag caataactag cat
//
