LOCUS       sequence-110936-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4R3H
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             588..605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(658..676)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcaccagta aactcaaata tgtgcttcaa gatgcaagat ttttcctcat aaagagtaac
      121 aaccatgaga atgtgtctct tgccaaagcg aagggtgtat ggtccacgct ccctgtaaat
      181 gagaagaaat taaatcttgc atttagatct gcaaggagtg ttatcttaat attttctgtc
      241 agagagagtg gaaaatttca agggtttgca agactttctt cagaatcaca tcacggagga
      301 tctcctatac actgggtgct tccagcagga atgagtgcta aaatgctggg aggtgtcttt
      361 aaaattgact ggatttgcag gcgtgaatta cccttcacta agtcggctca tctcaccaat
      421 ccttggaatg aacataaacc agtaaagatc ggacgtgatg gacaggaaat tgaacttgaa
      481 tgtggaaccc agctttgtct tctgtttccc cccgatgaaa gtattgactt gtatcaggtc
      541 attcataaaa tgcgtcactg atgacgaagc ttgcggccgc actcgagcac caccaccacc
      601 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      661 ctgagcaata actagcataa
//
