LOCUS       sequence-110937-        1305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses activated OTUB1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINB-OTUB1*
FEATURES             Location/Qualifiers
     source          1..1305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..63
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggtc tggaagttct gtttcagggc
       61 ccgatggctc tgaagagaat ccacaaggaa ttgaatgatc tggcacggga ccctccagca
      121 cagtgttcag caggtcctgt tggagatgat atgttccatt ggcaagctac aataatgggg
      181 ccaaatgaca gtccctatca gggtggagta tttttcttga caattcattt cccaacagat
      241 taccccttca aaccacctaa ggttgcattt acaacaagaa tttatcatcc aaatattaac
      301 agtaatggca gcattgctct tgatattcta cgatcacagt ggtctccagc actaactatt
      361 tcaaaagtac tcttgtccat ctgttctctg ttgtgtgatc ccaatccaga tgatccttta
      421 gtgcctgaga ttgctcggat ctacaaaaca gatagagaaa agtacaacag aatagctcgg
      481 gaatggactc agaagtatgc gatgggcggc agcagcggcg gcagcagcgg cggcagcgac
      541 tccgaaggtg ttaactgtct ggcctatgat gaagccatca tggctcagca ggaccgaatt
      601 cagcaagaga ttgctgtgca gaaccctctg gtgtcagagc ggctggagct ctcggtccta
      661 tacaaggagt atgctgaaga tgacaacatc tatcaacaga agatcaagga cctccacaaa
      721 aagtactcgt acatccgcaa gaccaggcct gacggcaact gtttctatcg ggctttcgga
      781 ttctcccact tggaggcact gctggatgac agcaaggagt tgcagcggtt caaggctgtg
      841 tctgccaaga gcaaggaaga cctggtgtcc cagggcttca ctgaattcac aattgaggat
      901 ttccacaaca cgttcatgga cctgattgag caggtggaga agcagacctc tgtcgccgac
      961 ctgctggcct ccttcaatga ccagagcacc tccgactacc ttgtggtcta cctgcggctg
     1021 ctcacctcgg gctacctgca gcgcgagagc aagttcttcg agcacttcat cgagggtgga
     1081 cggactgtca aggagttctg ccagcaggag gtggagccca tgtgcaagga gagcgaccac
     1141 atccacatca ttgcgctggc ccaggccctc agcgtgtcca tccaggtgga gtacatggac
     1201 cgcggcgagg gcggcaccac caatccgcac atcttccctg agggctccga gcccaaggtc
     1261 taccttctct accggcctgg acactacgat atcctctaca aataa
//
