LOCUS       sequence-110938-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  trp1 deletion gRNA cassette carried by pRS42H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: gRNA-trp-HYB
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
       61 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
      121 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg acctgcgagc agggaaacgc
      181 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
      241 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
      301 cagttctcac atcacatccg aacataaaca accatgggta aaaagcctga actcaccgcg
      361 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
      421 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
      481 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
      541 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
      601 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
      661 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
      721 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
      781 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
      841 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
      901 cccgaagtcc ggcacctcgt gcacgcg
//
