LOCUS       sequence-110946-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  leu2 deletion gRNA cassette carried by pRS42H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: gRNA-leu-HYB
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
       61 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
      121 ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc ggacctgcga gcagggaaac
      181 gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa
      241 ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga
      301 tacagttctc acatcacatc cgaacataaa caaccatggg taaaaagcct gaactcaccg
      361 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
      421 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
      481 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
      541 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
      601 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
      661 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
      721 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
      781 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
      841 acaccgtcag tgcgtccgtc gcgca
//
