LOCUS       sequence-110950-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For phage display of antibody libraries by fusion to modified 
            geneIII..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ
  TITLE     Assembly of combinatorial antibody libraries on phage surfaces: the 
            gene III site.
  JOURNAL   Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.
  PUBMED    1896445
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1991-09-15"; volume: "88(18)"; pages: 
            "7978-82"
COMMENT     Sequence Label: pComb3
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     52..117
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     131..147
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     132..148
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctggccacc gcggtggcgg ccgcaaattc tatttcaagg agacagtcat aatgaaatac
       61 ctattgccta cggcagccgc tggattgtta ttactcgctg cccaaccagc catggcccag
      121 gtgaaactgc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      181 ggatccacta gtggtggcgg tggctctcca ttcgtttgtg aatatcaagg ccaatcgtct
      241 gacctgcctc aacctcctgt caatgctggc ggcggctctg gtggtggttc tggtggcggc
      301 tctgagggtg gtggctctga gggtggcggt tctgagggtg gcggctctga gggaggcggt
      361 tccggtggtg gctctggttc cggtgatttt gattatgaaa agatggcaaa cgctaataag
      421 ggggctatga ccgaaaatgc cgatgaaaac gcgctacagt ctgacgctaa aggcaaactt
      481 gattctgtcg ctactgatta cggtgctgct atcgatggtt tcattggtga cgtttccggc
      541 cttgctaatg gtaatggtgc tactggtgat tttgctggct ctaattccca aatggctcaa
      601 gtcggtgacg gtgataattc acctttaatg aataatttcc gtcaatattt accttccctc
      661 cctcaatcgg ttgaatgtcg cccttttgtc tttggcgctg gtaaaccata tg
//
