LOCUS       sequence-110951-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSE401
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(307..327)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(334..399)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(553..599)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(588..608)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atcgtcaacc ttcgccatct cgttggagaa aatctcctgc aggtagcaga tgcgattctt
       61 cctgcgcgtg taccgcctgc gggcggtgcg cttgagccgc gtagcctcag ccgtctcgcc
      121 gctgtcgaac aggagagcgc caatgagatt cttcttgatg gaatgccgat cggtgttgcc
      181 caggaccttg aacttctttg agggcacctt gtactcgtcg gtgatcacgg cccagccaac
      241 agagttagtc ccaatatcga ggccgatcga gtacttcttg tcagcagctg gcaccccgtg
      301 gatgccaacc ttcctcttct tcttcggagc catcttgtca tcatcatcct tgtaatcaat
      361 gtcgtggtcc ttgtaatccc cgtcgtggtc cttgtaatcc atctagatta ctcgagtatc
      421 gttcgtaaat ggtgaaaatt ttcagaaaat tgcttttgct ttaaaagaaa tgatttaaat
      481 tgctgcaata gaagtagaat gcttgattgc ttgagattcg tttgttttgt atatgttgtg
      541 ttgaggtcga ggtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
      601 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
      661 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
      721 catctttggg accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat
      781 gatggcattt gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag
      841 ctgggcaatg gaatccgagg aggtttccgg atattaccct ttgttgaaa
//
