LOCUS       sequence-110954-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSE401
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(617..664)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             744..752
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aatgcattaa aaaatatttt catactcaac tacaaatcca tgagtataac tataattata
       61 aagcaatgat tagaatctga caaggattct ggaaaattac ataaaggaaa gttcataaat
      121 gtctaaaaca caagaggaca tacttgtatt cagtaacatt tgcagctttt ctaggtctga
      181 aaatatattt gttgcctagt gaataagcat aatggtacaa ctacaagtgt tttactcctc
      241 atattaactt cggtcattag aggccacgat ttgacacatt tttactcaaa acaaaatgtt
      301 tgcatatctc ttataatttc aaattcaaca cacaacaaat aagagaaaaa acaaataata
      361 ttaatttgag aatgaacaaa aggaccatat cattcattaa ctcttctcca tccatttcca
      421 tttcacagtt cgatagcgaa aaccgaataa aaaacacagt aaattacaag cacaacaaat
      481 ggtacaagaa aaacagtttt cccaatgcca taatactcaa actcagtagg attctggtgt
      541 gtgcgcaatg aaactgatgc attgaacttg acgaacgttg tcgaaaccga tgatacgaac
      601 gaaagctctg agctcacttc ttcttcttcg cctgccccgc cttcttcgtc gccgctggcc
      661 gcttgtcgcc cccgagctga gacaggtcga tgcgcgtctc gtagaggccg gtaatcgact
      721 ggtggatgag ggtcgcgtcc aggacctcct tagtgcttgt gtacctcttg cgatcga
//
