LOCUS       sequence-110957-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  his3 deletion gRNA cassette carried by pRS42H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: gRNA-his-HYB
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
       61 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      121 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg agcagggaaa
      181 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      241 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      301 atacagttct cacatcacat ccgaacataa acaaccatgg gtaaaaagcc tgaactcacc
      361 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
      421 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
      481 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
      541 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
      601 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
      661 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
      721 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
      781 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
      841 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgaa
//
