LOCUS       sequence-110958-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHUE411
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(667..714)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cagctatgac atgattacga attcgttgtc aatcaattgg caagtcataa aatgcattaa
       61 aaaatatttt catactcaac tacaaatcca tgagtataac tataattata aagcaatgat
      121 tagaatctga caaggattct ggaaaattac ataaaggaaa gttcataaat gtctaaaaca
      181 caagaggaca tacttgtatt cagtaacatt tgcagctttt ctaggtctga aaatatattt
      241 gttgcctagt gaataagcat aatggtacaa ctacaagtgt tttactcctc atattaactt
      301 cggtcattag aggccacgat ttgacacatt tttactcaaa acaaaatgtt tgcatatctc
      361 ttataatttc aaattcaaca cacaacaaat aagagaaaaa acaaataata ttaatttgag
      421 aatgaacaaa aggaccatat cattcattaa ctcttctcca tccatttcca tttcacagtt
      481 cgatagcgaa aaccgaataa aaaacacagt aaattacaag cacaacaaat ggtacaagaa
      541 aaacagtttt cccaatgcca taatactcaa actcagtagg attctggtgt gtgcgcaatg
      601 aaactgatgc attgaacttg acgaacgttg tcgaaaccga tgatacgaac gaaagctctg
      661 agctcacttc ttcttcttcg cctgccccgc cttcttcgtc gccgctggcc gcttgtcgcc
      721 cccgagctga gacaggtcga tgcgcgtctc gtagaggccg gtaatcgact ggtggatga
//
