LOCUS       sequence-110960-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone pComb3X containing PCR template for human antibody lambda 
            light chain constant region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd
  TITLE     Methods for the generation of chicken monoclonal antibody fragments 
            by phage display.
  JOURNAL   J Immunol Methods. 2000 Aug 28;242(1-2):159-81.
  PUBMED    10986398
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol 
            Methods."; date: "2000-08-28"; volume: "242(1-2)"; pages: "159-81"
COMMENT     Sequence Label: pComb3XLambda
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(685..711)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(718..735)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
       61 aattttaaca aaatattaac gcttacaatt tcccagatcc tcctcagaga gcagatctgc
      121 ggccgcttaa attaattagc tagcttaaga ctccttatta cgcagtatgt tagcaaacgt
      181 agaaaataca tacataaagg tggcaacata taaaagaaac gcaaagacac cacggaataa
      241 gtttattttg tcacaatcaa tagaaaattc atatggttta ccagcgccaa agacaaaagg
      301 gcgacattca accgattgag ggagggaagg taaatattga cggaaattat tcattaaagg
      361 tgaattatca ccgtcaccga cttgagccat ttgggaatta gagccagcaa aatcaccagt
      421 agcaccatta ccattagcaa ggccggaaac gtcaccaatg aaaccatcga tagcagcacc
      481 gtaatcagta gcgacagaat caagtttgcc tttagcgtca gactgtagca cgttttcatc
      541 ggcattttcg gtcatagccc ccttattagc gtttgccatc ttttcataat caaaatcacc
      601 ggaaccagag ccaccaccgg aaccgcctcc ctcagagccg ccaccctcag aaccgccacc
      661 ctcagagcca ccaccctcct aagaagcgta gtccggaacg tcgtacgggt atgcgccatg
      721 gtgatggtga tggtgct
//
