LOCUS       sequence-110977-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG201
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        113..463
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 gtcgaaagaa cgcggcatta gcaacatttg cgaagcgaac gattttgcga actgctatta
       61 attaaacaca caccatacct tcccatatgt cctggcgcgc ccttagctct cattattttt
      121 tgctttttct cttgaggtca catgatcgca aaatggcaaa tggcacgtga agctgtcgat
      181 attggggaac tgtggtggtt ggcaaatgac taattaagtt agtcaaggcg ccatcctcat
      241 gaaaactgtg taacataata accgaagtgt cgaaaaggtg gcaccttgtc caattgaaca
      301 cgctcgatga aaaaaataag atatatataa ggttaagtaa agcgtctgtt agaaaggaag
      361 tttttccttt ttcttgctct cttgtctttt catctactat ttccttcgtg taatacaggg
      421 tcgtcagata catagataca attctattac ccccatccat acaatgccat ctcatttcga
      481 tactgttcaa ctacacgccg gtcaagagaa ccctggtgac aatgctcaca gatccagagc
      541 tgtaccaatt tacgccacca cttcttatgt tttcgaaaac tctaagcatg gttcgcaatt
      601 gtttggttta gaagttccag gttacgtcta ttcccgtttc caaaacccaa ccagtaatgt
      661 tttggaagaa agaattgctg ctttagaagg tggtgctgct gctttggctg tttcctccgg
      721 tcaagccgct caaacccttg ccatccaagg tttggcacac actggtgaca acatcgtttc
      781 cacttcttac ttatacggtg gtacttataa ccagttcaaa atctcgttca aaagatttgg
      841 tatcgaggct agatttgttg aaggtgacaa tccagaagaa tttgaaaagg tctttgatga
      901 aagaaccaag gctgtttatt tggaaaccat tggtaatcca aagtacaatg ttccggattt
      961 tgaaaaaatt gttgcaattg ctcacaaaca cggtattcca gttgtcgttg acaacacatt
     1021 tggtgccggt ggttacttct gtcagccaat taaatacggt gctgatattg taacacattc
     1081 tgctaccaaa tggattggtg g
//
