LOCUS       sequence-110979-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG201
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     133..149
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     134..150
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(184..200)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(184..200)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(308..327)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(309..327)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(334..351)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(334..350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(343..365)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctgggt acaagtgaca
       61 atatatatat atatatataa taacgacatc gttatctgta aatacggccg aagctgggta
      121 ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg
      181 ggggatccac tagttctaga gccaccgcgg tggagctcca attcgcccta taggcggccg
      241 cgaaaagaaa tatagtcttc aaggttatat tatacaggtt tatatattat tctgtttctc
      301 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
      361 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatccga tcgtcaccaa
      421 ttccatttta tggattccta taccacgagg agacctagta tgtcctggct atctaatatt
      481 atggcctcta gcagcaacga aatgccaaca attaaatcca agttcaccta ttctgacttt
      541 tatatttaat aactaacatt tttatcaacg atggaattgc aacagcttta ctaatcttta
      601 cttgtaattt ggcttttttg atctataata tttattgtat ggaaagtagc atccaaccaa
      661 atcccgcgaa gttttcctga tttacataac aagcaaatac taaaaacgtt aacacacatg
      721 tgcttcttta ttgtaaagaa acctccatca tcctcttttg taacttggtc ctacaataaa
      781 tttatccagt gtgacagctt tataggaggc gtaaagtagt ctcatgaagt agatgagtct
      841 tgttcaccat atttttcttc tcgactgcga attaacactg ttctttgatg ttagaacaat
      901 ttaggttcaa agtacgagtc acgacatgta acaagggaaa aa
//
