LOCUS       sequence-110997-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG221
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     141..157
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     142..158
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(316..335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(317..335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(342..359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(342..358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(351..373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctgggtac
       61 aagtgacaat atatatatat atatataata acgacatcgt tatctgtaaa tacggccgaa
      121 gctgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
      181 gcagcccggg ggatccacta gttctagagc caccgcggtg gagctccaat tcgccctata
      241 ggcggccgcg aaaagaaata tagtcttcaa ggttatatta tacaggttta tatattattc
      301 tgtttctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
      361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccgatc
      421 gtcaccaatt ccattttatg gattcctata ccacgaggag acctagtatg tcctggctat
      481 ctaatattat ggcctctagc agcaacgaaa tgccaacaat taaatccaag ttcacctatt
      541 ctgactttta tatttaataa ctaacatttt tatcaacgat ggaattgcaa cagctttact
      601 aatctttact tgtaatttgg cttttttgat ctataatatt tattgtatgg aaagtagcat
      661 ccaaccaaat cccgcgaagt tttcctgatt tacataacaa gcaaatacta aaaacgttaa
      721 cacacatgtg cttctttatt gtaaagaaac ctccatcatc ctcttttgta acttggtcct
      781 acaataaatt tatccagtgt gacagcttta taggaggcgt aaagtagtct catgaagtag
      841 atgagtcttg ttcaccatat ttttcttctc gactgcgaat taacactgtt ctttgatgtt
      901 agaacaattt aggttcaaag tacgagtcac gacatgtaac aagggaaaaa
//
