LOCUS       sequence-111000-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG216
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    7..510
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
       61 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
      121 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
      181 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
      241 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
      301 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
      361 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
      421 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
      481 gaggcgcgtg taagttacag gcaagcgatc cgtcttaatt aactctggcg cgccactact
      541 ttttggaact cttgttgttc tttggagttc aatgcgtcca tctttacagt cctgtcttat
      601 tgttcttgat ttgtgccccg taaaatactg ttacttggtt ctggcgaggt attggatagt
      661 tcctttttat aaaggccatg aaggtttttc tttccaattt tttttttttc gtcattatag
      721 aaatcattac gaccgagatt cccggcatat ggtaataact gatataatta aattgaagct
      781 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
      841 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
      901 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
      961 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
     1021 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
     1081 gcaataaagc cgataacaaa
//
