LOCUS       sequence-111002-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Kan resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pKSE401
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(310..330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(337..402)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(556..602)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(591..611)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agaatcgtca accttcgcca tctcgttgga gaaaatctcc tgcaggtagc agatgcgatt
       61 cttcctgcgc gtgtaccgcc tgcgggcggt gcgcttgagc cgcgtagcct cagccgtctc
      121 gccgctgtcg aacaggagag cgccaatgag attcttcttg atggaatgcc gatcggtgtt
      181 gcccaggacc ttgaacttct ttgagggcac cttgtactcg tcggtgatca cggcccagcc
      241 aacagagtta gtcccaatat cgaggccgat cgagtacttc ttgtcagcag ctggcacccc
      301 gtggatgcca accttcctct tcttcttcgg agccatcttg tcatcatcat ccttgtaatc
      361 aatgtcgtgg tccttgtaat ccccgtcgtg gtccttgtaa tccatctaga ttactcgagt
      421 atcgttcgta aatggtgaaa attttcagaa aattgctttt gctttaaaag aaatgattta
      481 aattgctgca atagaagtag aatgcttgat tgcttgagat tcgtttgttt tgtatatgtt
      541 gtgttgaggt cgaggtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
      601 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
      661 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
      721 gtccatcttt gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc
      781 aatgatggca tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga
      841 tagctgggca atggaatccg aggaggtttc cggatattac cctt
//
