LOCUS       sequence-111005-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG216
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     141..157
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     142..158
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(316..335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(317..335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(342..359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(342..358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(351..373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        424..640
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctgggtac
       61 aagtgacaat atatatatat atatataata acgacatcgt tatctgtaaa tacggccgaa
      121 gctgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
      181 gcagcccggg ggatccacta gttctagagc caccgcggtg gagctccaat tcgccctata
      241 ggcggccgcg aaaagaaata tagtcttcaa ggttatatta tacaggttta tatattattc
      301 tgtttctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
      361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccgatc
      421 gcttttcatt catctttttt ttttttgttc ttttttttga ttccggtttc tttgaaattt
      481 ttttgattcg gtaatctccg agcagaagga agaacgaagg aaggagcaca gacttagatt
      541 ggtatatata cgcataggtg gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
      601 actgcacaga acaaaaacgt gcaggaaacg aagataaatc atgtcgaaag ctacatataa
      661 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
      721 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
      781 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggatc tcttgactga
      841 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
      901 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
      961 gtgtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     1021 aggtattgtt agcggtttga agc
//
