LOCUS       sequence-111011-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses destabilized Citrine in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: FRP1454
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(206..455)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     421..439
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctgggtac
       61 aagtgacaat atatatatat atatataata acgacatcgt tatctgtaaa tacggccgaa
      121 gctgggtacc aagcagacgc tactaaggaa aacaacaacg gaagcgatga tgagagcgac
      181 gatgagagtg taaactgcga accttgcaaa ttaaagcctt cgagcgtccc aaaaccttct
      241 caagcaaggt tttcagtata atgttacatg cgtacacgcg tttgtacaga aaaaaaagaa
      301 aaatttgaaa tataaataac gttcttaata ctaacataac tattaaaaaa aataaatagg
      361 gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatg tgggaggagg
      421 gcgtgaatgt aagcgtgaca taactaatta catgatatag acaaaggaaa aggggcctgt
      481 ctcgagttat ttagaagtgt caacaacgta tctaccaacg atttgaccct tttccatctt
      541 ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg attggagact tgaccaaacc
      601 tctggcgaag aagtccaaag ctctgaattg agtttccaaa ccggatcttc taacagtaga
      661 ccaagtggat cctttgtaca attcatccat accatgggta ataccagcag cagtaacaaa
      721 ttctaacaag accatgtggt ctctcttttc gtttggatct ttggataact tagattgata
      781 ggataagtaa tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg
      841 ataatggtca gctaattgaa cagaaccatc ttcaatgttg tgtctaattt tgaagttaac
      901 tttgatacca ttcttttgtt tgtcagccat gatgtaaaca ttgtgagagt tatagttgta
      961 ttccaatttg tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat
     1021 tctattaact aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc
     1081 atctttgaaa a
//
