LOCUS       sequence-111015-        6821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  containing 1.3 units of the X-null HBV genome (subtype ayw).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Hussain Z, Jung HS, Ryu DK, Ryu WS
  TITLE     Genetic dissection of naturally occurring basal core promoter 
            mutations of hepatitis B virus reveals a silent phenotype in the 
            overlapping X gene.
  JOURNAL   J Gen Virol. 2009 Sep;90(Pt 9):2272-81. doi: 10.1099/vir.0.010421-0.
            Epub 2009 May 13.
  PUBMED    19439550
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol."; date: "2009-09"; volume: "90(Pt 9):2272-81. doi"; pages: " 
            10.1099/vir.0.010421-0. Epub 2009 May 13"
COMMENT     Sequence Label: HBV 1.3-mer X-null replicon
FEATURES             Location/Qualifiers
     source          1..6821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1885..1904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1886..1904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1923..1939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1923..1939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1936..1958)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1947..1963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1971..1977,1978..1995,1996..2001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2016..2037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2154..2171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2325..2913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2405..2424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3084..3875,3876..3944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3707..3726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3945..4049)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4117..4135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4173..4195)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4295..4314
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4508..4530
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4522..4539
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4523..4539
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4547..4565
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4547..4564
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(6684..6821,1..708)
                     /codon_start=1
                     /product="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
                     /label=HBsAg (pre-S2/S)
                     /note="hepatitis B surface antigen"
                     /translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
                     FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
                     GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
                     LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
                     ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
                     "
ORIGIN
        1 tcgaggattg gggaccctgc gctgaacatg gagaacatca catcaggatt cctaggaccc
       61 cttctcgtgt tacaggcggg gtttttcttg ttgacaagaa tcctcacaat accgcagagt
      121 ctagactcgt ggtggacttc tctcaatttt ctagggggaa ctaccgtgtg tcttggccaa
      181 aattcgcagt ccccaacctc caatcactca ccaacctctt gtcctccaac ttgtcctggt
      241 tatcgctgga tgtgtctgcg gcgttttatc atcttcctct tcatcctgct gctatgcctc
      301 atcttcttgt tggttcttct ggactatcaa ggtatgttgc ccgtttgtcc tctaattcca
      361 ggatcctcaa caaccagcac gggaccatgc cggacctgca tgactactgc tcaaggaacc
      421 tctatgtatc cctcctgttg ctgtaccaaa ccttcggacg gaaattgcac ctgtattccc
      481 atcccatcat cctgggcttt cggaaaattc ctatgggagt gggcctcagc ccgtttctcc
      541 tggctcagtt tactagtgcc atttgttcag tggttcgtag ggctttcccc cactgtttgg
      601 ctttcagtta tatggatgat gtggtattgg gggccaagtc tgtacagcat cttgagtccc
      661 tttttaccgc tgttaccaat tttcttttgt ctttgggtat acatttaaac cctaacaaaa
      721 caaagagatg gggttactct ctaaatttta tgggttatgt cattggatgt tatgggtcct
      781 tgccacaaga acacatcata caaaaaatca aagaatgttt tagaaaactt cctattaaca
      841 ggcctattga ttggaaagta tgtcaacgaa ttgtgggtct tttgggtttt gctgcccctt
      901 ttacacaatg tggttatcct gcgttgatgc ctttgtatgc atgtattcaa tctaagcagg
      961 ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa acaatacctg aacctttacc
     1021 ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc tgacgcaacc cccactggct
     1081 ggggcttggt catgggccat cagcgcatgc gtggaacctt ttcggctcct ctgccgatcc
     1141 atactgcgga actcctagcc gcttgttttg ctcgcagcag gtctggagca aacattatcg
     1201 ggactgataa ctctgttgtc ctatcccgca aatatacatc gtttccatgg ctgctaggct
     1261 gtgctgctaa ctggatcctg cgcgggacgt cctttgttta cgtcccgtcg gcgctgaatc
     1321 ctgcggacga cccttctcgg ggtcgcttgg gactctctcg tccccttctc cgtctgccgt
     1381 tccgaccgac cacggggcgc acctctcttt acgcggactc cccgtctgtg ccttctcatc
     1441 tgccggaccg tgtgcacttc gcttcacctc tgcacgtcgc atggagacca ccgtgaacgc
     1501 ccactaaata ttgcccaagg tcttacataa gaggactctt ggactctcag caatgtcaac
     1561 gaccgacctt gaggcatact tcaaagactg tttgtttaaa gactgggagg agttggggga
     1621 ggagattagg ttaaaggtct ttgtactagg aggctgtagg cataaattgg tctgcgcacc
     1681 agcaccatgc aactttttca cctctgccta atcatctctt gttcatgtcc tactgttcaa
     1741 gcctccaagc tgtgccttgg gtggctttgg ggcatggaca tcgaccctta taaagaattt
     1801 ggagctactg tggagttact ctcgtttttg ccttctgact tctttccttc agtacgagat
     1861 cttctagata ccgcaagctt gtctccctat agtgagtcgt attagagctt ggcgtaatca
     1921 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1981 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2041 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2101 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2161 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2221 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2281 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2341 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2401 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2461 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2521 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2581 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2641 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2701 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2761 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2821 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2881 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2941 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3001 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3061 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3121 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3181 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3241 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3301 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3361 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3421 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3481 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3541 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3601 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3661 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3721 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3781 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3841 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3901 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3961 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4021 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4081 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4141 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     4201 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     4261 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     4321 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     4381 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4441 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     4501 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattggattt aggtgacact
     4561 atagaatacg aattcgagct cgtattcaat ctaagcaggc tttcactttc tcgccaactt
     4621 acaaggcctt tctgtgtaaa caatacctga acctttaccc cgttgcccgg caacggccag
     4681 gtctgtgcca agtgtttgct gacgcaaccc ccactggctg gggcttggtc atgggccatc
     4741 agcgcatgcg tggaaccttt tcggctcctc tgccgatcca tactgcggaa ctcctagccg
     4801 cttgttttgc tcgcagcagg tctggagcaa acattatcgg gactgataac tctgttgtcc
     4861 tatcccgcaa atatacatcg tttccatggc tgctaggctg tgctgccaac tggatcctgc
     4921 gcgggacgtc ctttgtttac gtcccgtcgg cgctgaatcc tgcggacgac ccttctcggg
     4981 gtcgcttggg actctctcgt ccccttctcc gtctgccgtt ccgaccgacc acggggcgca
     5041 cctctcttta cgcggactcc ccgtctgtgc cttctcatct gccggaccgt gtgcacttcg
     5101 cttcacctct gcacgtcgca tggagaccac cgtgaacgcc cactaaatat tgcccaaggt
     5161 cttacataag aggactcttg gactctcagc aatgtcaacg accgaccttg aggcatactt
     5221 caaagactgt ttgtttaaag actgggagga gttgggggag gagattaggt taaaggtctt
     5281 tgtactagga ggctgtaggc ataaattggt ctgcgcacca gcaccatgca actttttcac
     5341 ctctgcctaa tcatctcttg ttcatgtcct actgttcaag cctccaagct gtgccttggg
     5401 tggctttggg gcatggacat cgacccttat aaagaatttg gagctactgt ggagttactc
     5461 tcgtttttgc cttctgactt ctttccttca gtacgagatc ttctagatac cgcctcagct
     5521 ctgtatcggg aagccttaga gtctcctgag cattgttcac ctcaccatac tgcactcagg
     5581 caagcaattc tttgctgggg ggaactaatg actctagcta cctgggtggg tgttaatttg
     5641 gaagatccag cgtctagaga cctagtagtc agttatgtca acactaatat gggcctaaag
     5701 ttcaggcaac tcttgtggtt tcacatttct tgtctcactt ttggaagaga aacagttata
     5761 gagtatttgg tgtctttcgg agtgtggatt cgcactcctc cagcttatag accaccaaat
     5821 gcccctatcc tatcaacact tccggagact actgttgtta gacgacgagg caggtcccct
     5881 agaagaagaa ctccctcgcc tcgcagacga aggtctcaat cgccgcgtcg cagaagatct
     5941 caatctcggg aatctcaatg ttagtattcc ttggactcat aaggtgggga actttactgg
     6001 gctttattct tctactgtac ctgtctttaa tcctcattgg aaaacaccat cttttcctaa
     6061 tatacattta caccaagaca ttatcaaaaa atgtgaacag tttgtaggcc cactcacagt
     6121 taatgagaaa agaagattgc aattgattat gcctgccagg ttttatccaa aggttaccaa
     6181 atatttacca ttggataagg gtattaaacc ttattatcca gaacatctag ttaatcatta
     6241 cttccaaact agacactatt tacacactct atggaaggcg ggtatattat ataagagaga
     6301 aacaacacat agcgcctcat tttgtgggtc accatattct tgggaacaag atctacagca
     6361 tggggcagaa tctttccacc agcaatcctc tgggattctt tcccgaccac cagttggatc
     6421 cagccttcag agcaaacacc gcaaatccag attgggactt caatcccaac aaggacacct
     6481 ggccagacgc caacaaggta ggagctggag cattcgggct gggtttcacc ccaccgcacg
     6541 gaggcctttt ggggtggagc cctcaggctc agggcatact acaaactttg ccagcaaatc
     6601 cgcctcctgc ctccaccaat cgccagtcag gaaggcagcc taccccgctg tctccacctt
     6661 tgagaaacac tcatcctcag gccatgcagt ggaattccac aaccttccac caaactctgc
     6721 aagatcccag agtgagaggc ctgtatttcc ctgctggtgg ctccagttca ggaacagtaa
     6781 accctgttct gactactgcc tctcccttat cgtcaatctt c
//
