LOCUS       sequence-111016-        6820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  containing 1.3 units of the P-null HBV genome (subtype ayw).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Wang H, Kim S, Ryu WS
  TITLE     DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse 
            transcription by incorporation into nucleocapsids.
  JOURNAL   J Virol. 2009 Jun;83(11):5815-24. doi: 10.1128/JVI.00011-09. Epub 
            2009 Mar 18.
  PUBMED    19297497
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00011-09"; journalName: "J Virol"; date: "2009-06"; 
            volume: "83"; issue: "11"; pages: "5815-24"
COMMENT     Sequence Label: HBV 1.3-mer P-null replicon
FEATURES             Location/Qualifiers
     source          1..6820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             842..1687
                     /codon_start=1
                     /product="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
                     /label=HBsAg (pre-S2/S)
                     /note="hepatitis B surface antigen"
                     /translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
                     FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
                     GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
                     LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
                     ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
                     "
     primer_bind     complement(2864..2883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2865..2883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2902..2918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2902..2918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2915..2937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2926..2942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2950..2956,2957..2974,2975..2980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2995..3016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3133..3150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3304..3892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3384..3403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4063..4854,4855..4923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4686..4705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4924..5028)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5096..5114
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5152..5174)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5274..5293
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5487..5509
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5501..5518
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5502..5518
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5526..5544
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5526..5543
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccggagacta ctgttgttag acgacgaggc aggtccccta gaagaagaac tccctcgcct
       61 cgcagacgaa ggtctcaatc gccgcgtcgc agaagatctc aatctcggga atctcaatgt
      121 tagtattcct tggactcata aggtggggaa ctttactggg ctttattctt ctactgtacc
      181 tgtctttaat cctcattgga aaacaccatc ttttcctaat atacatttac accaagacat
      241 tatcaaaaaa tgtgaacagt ttgtaggccc actcacagtt aatgagaaaa gaagattgca
      301 attgattagc ctgccaggtt ttatccaaag gttaccaaat atttaccatt ggataagggt
      361 attaaacctt attatccaga acatctagtt aatcattact tccaaactag acactattta
      421 cacactctat ggaaggcggg tatattatat aagagagaaa caacacatag cgcctcattt
      481 tgtgggtcac catattcttg ggaacaagat ctacagcatg gggcagaatc tttccaccag
      541 caatcctctg ggattctttc ccgaccacca gttggatcca gccttcagag caaacaccgc
      601 aaatccagat tgggacttca atcccaacaa ggacacctgg ccagacgcca acaaggtagg
      661 agctggagca ttcgggctgg gtttcacccc accgcacgga ggccttttgg ggtggagccc
      721 tcaggctcag ggcatactac aaactttgcc agcaaatccg cctcctgcct ccaccaatcg
      781 ccagtcagga aggcagccta ccccgctgtc tccacctttg agaaacactc atcctcaggc
      841 catgcagtgg aattccacaa ccttccacca aactctgcaa gatcccagag tgagaggcct
      901 gtatttccct gctggtggct ccagttcagg aacagtaaac cctgttctga ctactgcctc
      961 tcccttatcg tcaatcttct cgaggattgg ggaccctgcg ctgaacatgg agaacatcac
     1021 atcaggattc ctaggacccc ttctcgtgtt acaggcgggg tttttcttgt tgacaagaat
     1081 cctcacaata ccgcagagtc tagactcgtg gtggacttct ctcaattttc tagggggaac
     1141 taccgtgtgt cttggccaaa attcgcagtc cccaacctcc aatcactcac caacctcttg
     1201 tcctccaact tgtcctggtt atcgctggat gtgtctgcgg cgttttatca tcttcctctt
     1261 catcctgctg ctatgcctca tcttcttgtt ggttcttctg gactatcaag gtatgttgcc
     1321 cgtttgtcct ctaattccag gatcctcaac aaccagcacg ggaccatgcc ggacctgcat
     1381 gactactgct caaggaacct ctatgtatcc ctcctgttgc tgtaccaaac cttcggacgg
     1441 aaattgcacc tgtattccca tcccatcatc ctgggctttc ggaaaattcc tatgggagtg
     1501 ggcctcagcc cgtttctcct ggctcagttt actagtgcca tttgttcagt ggttcgtagg
     1561 gctttccccc actgtttggc tttcagttat atggatgatg tggtattggg ggccaagtct
     1621 gtacagcatc ttgagtccct ttttaccgct gttaccaatt ttcttttgtc tttgggtata
     1681 catttaaacc ctaacaaaac aaagagatgg ggttactctc taaattttat gggttatgtc
     1741 attggatgtt atgggtcctt gccacaagaa cacatcatac aaaaaatcaa agaatgtttt
     1801 agaaaacttc ctattaacag gcctattgat tggaaagtat gtcaacgaat tgtgggtctt
     1861 ttgggttttg ctgccccttt tacacaatgt ggttatcctg cgttgatgcc tttgtatgca
     1921 tgtattcaat ctaagcaggc tttcactttc tcgccaactt acaaggcctt tctgtgtaaa
     1981 caatacctga acctttaccc cgttgcccgg caacggccag gtctgtgcca agtgtttgct
     2041 gacgcaaccc ccactggctg gggcttggtc atgggccatc agcgcatgcg tggaaccttt
     2101 tcggctcctc tgccgatcca tactgcggaa ctcctagccg cttgttttgc tcgcagcagg
     2161 tctggagcaa acattatcgg gactgataac tctgttgtcc tatcccgcaa atatacatcg
     2221 tttccatggc tgctaggctg tgctgccaac tggatcctgc gcgggacgtc ctttgtttac
     2281 gtcccgtcgg cgctgaatcc tgcggacgac ccttctcggg gtcgcttggg actctctcgt
     2341 ccccttctcc gtctgccgtt ccgaccgacc acggggcgca cctctcttta cgcggactcc
     2401 ccgtctgtgc cttctcatct gccggaccgt gtgcacttcg cttcacctct gcacgtcgca
     2461 tggagaccac cgtgaacgcc caccaaatat tgcccaaggt cttacataag aggactcttg
     2521 gactctcagc aatgtcaacg accgaccttg aggcatactt caaagactgt ttgtttaaag
     2581 actgggagga gttgggggag gagattaggt taaaggtctt tgtactagga ggctgtaggc
     2641 ataaattggt ctgcgcacca gcaccatgca actttttcac ctctgcctaa tcatctcttg
     2701 ttcatgtcct actgttcaag cctccaagct gtgccttggg tggctttggg gcatggacat
     2761 cgacccttat aaagaattcg gagctactgt ggagttactc tcgtttttgc cttctgactt
     2821 ctttccttca gtacgagatc ttctagatac cgcaagcttg tctccctata gtgagtcgta
     2881 ttagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2941 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3001 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3061 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3121 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3721 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3961 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4021 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4081 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4141 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4201 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4261 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4321 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4381 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4441 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4501 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4561 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4621 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4681 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4741 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4801 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4861 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4921 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4981 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5041 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5101 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     5161 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     5221 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     5281 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5341 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5401 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5461 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     5521 attggattta ggtgacacta tagaatacga attcgagctc gtattcaatc taagcaggct
     5581 ttcactttct cgccaactta caaggccttt ctgtgtaaac aatacctgaa cctttacccc
     5641 gttgcccggc aacggccagg tctgtgccaa gtgtttgctg acgcaacccc cactggctgg
     5701 ggcttggtca tgggccatca gcgcatgcgt ggaacctttt cggctcctct gccgatccat
     5761 actgcggaac tcctagccgc ttgttttgct cgcagcaggt ctggagcaaa cattatcggg
     5821 actgataact ctgttgtcct atcccgcaaa tatacatcgt ttccatggct gctaggctgt
     5881 gctgccaact ggatcctgcg cgggacgtcc tttgtttacg tcccgtcggc gctgaatcct
     5941 gcggacgacc cttctcgggg tcgcttggga ctctctcgtc cccttctccg tctgccgttc
     6001 cgaccgacca cggggcgcac ctctctttac gcggactccc cgtctgtgcc ttctcatctg
     6061 ccggaccgtg tgcacttcgc ttcacctctg cacgtcgcat ggagaccacc gtgaacgccc
     6121 actaaatatt gcccaaggtc ttacataaga ggactcttgg actctcagca atgtcaacga
     6181 ccgaccttga ggcatacttc aaagactgtt tgtttaaaga ctgggaggag ttgggggagg
     6241 agattaggtt aaaggtcttt gtactaggag gctgtaggca taaattggtc tgcgcaccag
     6301 caccatgcaa ctttttcacc tctgcctaat catctcttgt tcatgtccta ctgttcaagc
     6361 ctccaagctg tgccttgggt ggctttgggg catggacatc gacccttata aagaatttgg
     6421 agctactgtg gagttactct cgtttttgcc ttctgacttc tttccttcag tacgagatct
     6481 tctagatacc gcctcagctc tgtatcggga agccttagag tctcctgagc attgttcacc
     6541 tcaccatact gcactcaggc aagcaattct ttgctggggg gaactaatga ctctagctac
     6601 ctgggtgggt gttaatttgg aagatccagc gtctagagac ctagtagtca gttatgtcaa
     6661 cactaatatg ggcctaaagt tcaggcaact cttgtggttt cacatttctt gtctcacttt
     6721 tggaagagaa acagttatag agtatttggt gtctttcgga gtgtggattc gcactcctcc
     6781 agcttataga ccaccaaacg cccctatcct atcaacactt
//
