LOCUS       sequence-111029-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for a sgRNA against the mouse Rosa26 locus and 
            Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcaggtgacg tggaggagaa tcccgggcct cttaagatgg agagccgaaa tccttttcag
       61 cagggactgc cagccgggtt cctgtcttca tcctttgtgg agaatatgga ggtgccagcc
      121 cccgagtgca acctgcgact gctggcagga accgcagctc gccacagtga ggatcctgaa
      181 tcaccagcag ccggcggaag taggagagag agtgaatcac gaccaggacc ttcaggagga
      241 ggagtcgctg acctgttccc agaactgcat cgcgtgctga cacgaagctc ctctggccga
      301 gagaggggaa tcaagagaga gcggcacgat gaaaccaatc ataggattga gctgacagtg
      361 gggctgatgt ctcgcaaacg acctgagact gtctggtggt acgaagtgca gagcaccggc
      421 acagacgaag tgtccgtcat gcacgagagg ttttctctgg aacaggtgaa gacctgttgg
      481 ctggagcctg aagacgattg ggaggtggcc atcagaaact acgccaagct ggctctgaga
      541 ccagaccgga agtacaagat cactaagctg atcaacatcc ggaatgcttg ctatatcagc
      601 ggcaatggag cagaggtcga aatttgtctg caggataggg tggccttcag atgctgtatg
      661 atgaacatgt accccggggt ggtcgacatg gatggcgtga ctttcatgaa tatga
//
