LOCUS       sequence-111030-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for a sgRNA against the mouse Rosa26 locus and 
            Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcttgacccg aactcagtgc cagtgcagga caggaccaga tgatcagggc gcagatcctc
       61 tgtcacgtcc acgcacacgg gctgaaaccg agcgtgcttc cctccacact cgcaggccct
      121 gcatcgagtt ttatattcgt cgtagcgcag gatcttccag acctccacat tcatatcgaa
      181 cacgcctgtc agagacaccc gggacatcac gtcgggttcc agcaggactt tcacataatt
      241 caggttacac tggtatggca taaacatgcc gcgccgggcc cccagatgca tattgcatct
      301 agtcatcacg ttgtgttcga actcgggcca tggctttcga ctatgagaag cgacgtgcac
      361 tgcagccagc atatgactgt tgcccccggc acatgtcagc atctggtacc ctctctcgtc
      421 agaggctccg caaatcatat tgtgtttgat ctttgcgttt cctttgcaca gcacgaagca
      481 gccggtctca gtggaggcac aatggcgaat ccgtgcttct ccctcgctca tcacgcccag
      541 gtgacatctt tcaaacaggc atttcttcac gctcagcatt gattttgtcc tcccgaccac
      601 tcccatccag tttgcggaga agctgcagcc cttgaccccc acttgtcccc aagcctcgat
      661 acaggtattg ttaaacccaa agaaggagca tccatgcaca gtcagctttg tattagccat
      721 gaagacggtg ccgttatagc catc
//
