LOCUS       sequence-111035-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for repair of the traffic light reporter (TLR), providing the
            venus coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pTLR repair vector
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..734)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 tcatttacgc gtgcgatcgc tcaattaagt ttgtgcccca gtttgctagg gaggtcgcag
       61 tatctggcca cagccacctc gtgctgctcg acgtaggtct ctttgtcggc ctccttgatt
      121 ctttccagtc tgtggtccac atagtagacg ccgggcatct tgaggttctt agcgggtttc
      181 ttggatctgt atgtggtctt gaagttgcag atcaggtggc ccccgcccac gagcttcagg
      241 gccatgtcgc ttctgccttc caggccgccg tcagcggggt acagcatctc ggtgttggcc
      301 tcccagccga gtgttttctt ctgcatcaca gggccgttgg atgggaagtt cacccctctg
      361 atcttgacgt tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc
      421 acgcccccgt cttcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc
      481 ttaaagaagt cggggatgcc ctgggtgtgg ttgatgaagg ttctgctgcc gtacatgaag
      541 ctggtagcca ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc
      601 atggtctggg tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtggtgg
      661 ttgttcacgg tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcttcg
      721 cccttagaca ccata
//
