LOCUS       sequence-111037-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG203MX
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             270..920
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
ORIGIN
        1 gtcgaaagaa cgcggcatta gcaacatttg cgaagcgaac gattttgcga actgctatta
       61 attaactctg gcgcgcctcc tctagtacac tctatatttt tttatgcctc ggtaatgatt
      121 ttcatttttt tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat
      181 cacataatga attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa
      241 atgagcaggc aagataaacg aaggcacata tgaggagggc ttttgtagaa agaaatacga
      301 acgaaacgaa aatcagcgtt gccatcgctt tggacaaagc tcccttacct gaagagtcga
      361 attttattga tgaacttata acttccaagc atgcaaacca aaagggagaa caagtaatcc
      421 aagtagacac gggaattgga ttcttggatc acatgtatca tgcactggct aaacatgcag
      481 gctggagctt acgactttac tcaagaggtg atttaatcat cgatgatcat cacactgcag
      541 aagatactgc tattgcactt ggtattgcat tcaagcaggc tatgggtaac tttgccggtg
      601 ttaaaagatt tggacatgct tattgtccac ttgacgaagc tctttccaga agcgtagttg
      661 acttgtcggg acggccctat gctgttatcg atttgggatt aaagcgtgaa aaggttgggg
      721 aattgtcctg tgaaatgatc cctcacttac tatattcctt ttcggtagct gctggaatta
      781 ctttgcatgt tacctgctta tatggtagta atgaccatca tcgtgctgaa agcgctttta
      841 aatctctggc tgttgccatg cgcgcggcta cttctcttac tggaagttct gaagtcccaa
      901 gcacgaaggg agtgttgtaa agatgaattg gattatgtca ggaaaagaac gacaattttg
      961 catccaaatt gtctaaattt tagagttgct tgaaaacaat agaaccttac ttgctttata
     1021 attacgttaa ttagaagcgt tatctcgtga aggaatatag tacgtagccg tataaattga
     1081 attgaatgtt cagcttatag aatagagaca ctt
//
