LOCUS       sequence-111052-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG215
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..514
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
       61 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
      121 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
      181 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
      241 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
      301 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
      361 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
      421 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
      481 atacgaggcg cgtgtaagtt acaggcaagc gatccgtctt aattaactct ggcgcgccta
      541 actttttgtg tggtgccctc ctccttgtca atattaatgt taaagtgcaa ttctttttcc
      601 ttatcacgtt gagccattag tatcaatttg cttacctgta ttcctttaca tcctcctttt
      661 tctccttctt gataaatgta tgtagattgc gtatatagtt tcgtctaccc tatgaacata
      721 ttccattttg taatttcgtg tcgcatatgt ttctattatg aatttcattt ataaagttta
      781 tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg
      841 cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac
      901 ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca
      961 atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg accttattct
     1021 ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt
     1081 ctggcaaaga
//
