LOCUS       sequence-111053-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pDEST26 Ago2 S387A
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      723..732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatatacga ctcactatag ggagagagct atgacgtcgc atgcacgcga tcaaccactt
       61 tgtacaagaa agctgggttc aagcaaagta catggtgcgc agagtgtctt ggtgaacctg
      121 gaccgccttg gccagtgctt ggtggtctcg cccgttactc tgcccagagg tatggcttcc
      181 ttcagcactg tcatgttcct tatccaccag gtggtacctg gcccggaagg ccaccaggtg
      241 agcgtagtat gctggcgctg ggatggacac ggagcgtgtg cagcgcacgt aggtgtgaca
      301 cagctggtag gttaggatct gcagctcatc agaggagaaa cgattgtcgt cccagaggac
      361 gtgatagtgc gaaggcctgc ttgtcccctg gatgccagcg tgactacaca ggtagaagtc
      421 gaactctgtg gggtgggtga ttttcgtgtc cacagtcgtg cctgctggaa tgtttccact
      481 tttcccaacc cgctcgttct tgtcagtgca gaagagccgg gtgtggtgcc tcttctgcac
      541 cacgatgaag gtgatccctg gctggtagtc tttttctagc ttgatacagg cctcacggat
      601 ggccagcaac tcgtggtgga gaacctgctg gaactggcct tcagagacac cgtcgcggta
      661 gaagatgatg cgggtgggct tgaagcgcgt ggacttgtag aactggatga ggagctcgcg
      721 gaccatggcg gccaggtctt gtatgatctc ctgccggtgc tgctgcacac gcacggtggc
      781 gcagtagcga ttggggtggg cgtccatgct gcccaccacg gcggcaatgg agggcttctt
      841 cccatccccg gcgggggggt gagtgacgtc tgctcccaga aagatgacgg gctgctggaa
      901 caccggcggc tg
//
