LOCUS       sequence-111054-        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG215
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     140..156
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     141..157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(341..358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(341..357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(350..372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        568..973
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
      181 cagcccgggg gatccactag ttctagagcc accgcggtgg agctccaatt cgccctatag
      241 gcggccgcga aaagaaatat agtcttcaag gttatattat acaggtttat atattattct
      301 gtttctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccgatcg
      421 aaaatcaatt gtcctgtact tccttgttca tgtgtgttca aaaacgttat atttatagga
      481 taattatact ctatttctca acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct
      541 gattcaagaa atatcttgac cgcagttaac tgtgggaata ctcaggtatc gtaagatgca
      601 agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg
      661 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc
      721 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg
      781 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac
      841 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg
      901 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatttcaagg
      961 atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg
     1021 tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa
     1081 tgtcaagttc gatttccaaa atcatttaat tggtggtgct gctatc
//
