LOCUS       sequence-111056-        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG206MX
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     140..156
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     141..157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(341..358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(341..357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(350..372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        460..803
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
      181 cagcccgggg gatccactag ttctagagcc accgcggtgg agctccaatt cgccctatag
      241 gcggccgcga aaagaaatat agtcttcaag gttatattat acaggtttat atattattct
      301 gtttctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccgatcg
      421 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
      481 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
      541 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
      601 cgaagcaaaa attacggctc ctcgctgcgg acctgcgagc agggaaacgc tcccctcaca
      661 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
      721 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
      781 atcacatccg aacataaaca accatgacag tcaacactaa gacctatagt gagagagcag
      841 aaactcatgc ctcaccagta gcacaacgat tatttcgatt aatggaactg aagaaaacca
      901 atttatgtgc atcaattgat gttgatacca ctaaggaatt tcttgaatta attgataaat
      961 tgggtcctta tgtatgctta atcaagactc atattgatat aatcaatgat ttttcctatg
     1021 aatccactat tgaaccatta ttagaacttt cacgtaaaca tcaatttatg atttttgaag
     1081 atagaaaatt tgctgatatt ggtaataccg tgaagaaaca a
//
