LOCUS       sequence-111059-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG236
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(298..1101)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDLLTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSACVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gtcgaaagaa cgcggcatta gcaacatttg cgaagcgaac gattttgcga actgctatta
       61 attaactctg gcgcgccact atcgtctatc aactaatagt tatattatca atatattatc
      121 atatacggtg ttaagatgat gacataagtt atgagaagct gtcatcgaag ttagaggaag
      181 ctgaagtgca aggattgata atgtaatagg ataatcatat ggtaataact gatataatta
      241 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
      301 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
      361 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
      421 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
      481 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
      541 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
      601 acccttagta tattctccag tagctaggga gcccttgcat gacaattctg ctaacatcaa
      661 aaggcctcta ggttcctttg ttacttcttc cgccgcctgc ttcaaaccgc taacaatacc
      721 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacaca
      781 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
      841 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
      901 atcagtcaag agatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
      961 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     1021 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     1081 cttatatgta gctttcgaca tgatt
//
