LOCUS       sequence-111061-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG236
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     140..156
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     141..157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(341..358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(341..357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(350..372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
      181 cagcccgggg gatccactag ttctagagcc accgcggtgg agctccaatt cgccctatag
      241 gcggccgcga aaagaaatat agtcttcaag gttatattat acaggtttat atattattct
      301 gtttctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccgatcg
      421 aacccaactg cacagaacaa aaacgtgcag gaaacgaaga taaatcatgt cgaaagctac
      481 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
      541 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
      601 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatctctt
      661 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
      721 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
      781 ctctgcgtgt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
      841 gggcccaggt attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag
      901 aggccttttg atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac
      961 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
     1021 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
     1081 tt
//
