LOCUS       sequence-111062-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG211
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..514
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        574..924
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
       61 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
      121 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
      181 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
      241 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
      301 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
      361 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
      421 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
      481 atacgaggcg cgtgtaagtt acaggcaagc gatccgtctt aattaaacac acaccatacc
      541 ttcccatatg tcctggcgcg cccttagctc tcattatttt ttgctttttc tcttgaggtc
      601 acatgatcgc aaaatggcaa atggcacgtg aagctgtcga tattggggaa ctgtggtggt
      661 tggcaaatga ctaattaagt tagtcaaggc gccatcctca tgaaaactgt gtaacataat
      721 aaccgaagtg tcgaaaaggt ggcaccttgt ccaattgaac acgctcgatg aaaaaaataa
      781 gatatatata aggttaagta aagcgtctgt tagaaaggaa gtttttcctt tttcttgctc
      841 tcttgtcttt tcatctacta tttccttcgt gtaatacagg gtcgtcagat acatagatac
      901 aattctatta cccccatcca tacaatgcca tctcatttcg atactgttca actacacgcc
      961 ggtcaagaga accctggtga caatgctcac agatccagag ctgtaccaat ttacgccacc
     1021 acttcttatg ttttcgaaaa ctctaagcat ggttcgcaat tgtttggttt agaagttcca
     1081 ggttacgtct attcccgttt ccaaaaccca accagtaatg ttttggaaga aagaattgct
     1141 gctt
//
