LOCUS       sequence-111064-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG211
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     140..156
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     141..157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(341..358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(341..357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(350..372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
      181 cagcccgggg gatccactag ttctagagcc accgcggtgg agctccaatt cgccctatag
      241 gcggccgcga aaagaaatat agtcttcaag gttatattat acaggtttat atattattct
      301 gtttctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccgatcg
      421 tcaccaattc cattttatgg attcctatac cacgaggaga cctagtatgt cctggctatc
      481 taatattatg gcctctagca gcaacgaaat gccaacaatt aaatccaagt tcacctattc
      541 tgacttttat atttaataac taacattttt atcaacgatg gaattgcaac agctttacta
      601 atctttactt gtaatttggc ttttttgatc tataatattt attgtatgga aagtagcatc
      661 caaccaaatc ccgcgaagtt ttcctgattt acataacaag caaatactaa aaacgttaac
      721 acacatgtgc ttctttattg taaagaaacc tccatcatcc tcttttgtaa cttggtccta
      781 caataaattt atccagtgtg acagctttat aggaggcgta aagtagtctc atgaagtaga
      841 tgagtcttgt tcaccatatt tttcttctcg actgcgaatt aacactgttc tttgatgtta
      901 gaacaattta ggttcaaagt acgagtcacg acatgtaaca agggaaaa
//
