LOCUS       sequence-111067-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pDEST26 Ago2 4A
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    152..176
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
       61 agaagacacc gggaccgatc cagcctccgg actctagcct aggccgcgga ccatggcgta
      121 ctaccatcac catcaccatc actctagatc aacaagtttg tacaaaaaag caggcttaat
      181 gtactcggga gccggccccg cacttgcacc tcctgcgccg ccgcccccca tccaaggata
      241 tgccttcaag cctccaccta gacccgactt tgggacctcc gggagaacaa tcaaattaca
      301 ggccaatttc ttcgaaatgg acatccccaa aattgacatc tatcattatg aattggatat
      361 caagccagag aagtgcccga ggagagttaa cagggaaatc gtggaacaca tggtccagca
      421 ctttaaaaca cagatctttg gggatcggaa gcccgtgttt gacggcagga agaatctata
      481 cacagccatg ccccttccga ttgggaggga caaggtggag ctggaggtca cgctgccagg
      541 agaaggcaag gatcgcatct tcaaggtgtc catcaagtgg gtgtcctgcg tgagcttgca
      601 ggcgttacac gatgcacttt cagggcggct gcccagcgtc ccttttgaga cgatccaggc
      661 cctggacgtg gtcatgaggc acttgccatc catgaggtac acccccgtgg gccgctcctt
      721 cttcaccgcg tccgaaggct gctctaaccc tcttggcggg ggccgagaag tgtggtttgg
      781 cttccatcag tccgtccggc cttctctctg gaaaatgatg ctgaatattg atgtgtcagc
      841 aacagcgttt tacaaggcac agccagtaat cgagtttgtt tgtgaagttt tggattttaa
      901 aagtattgaa gaacaacaaa aacctctgac agattcccaa agggtaaagt ttaccaaaga
      961 aattaa
//
