LOCUS       sequence-111068-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pDEST26 Ago2 4A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      722..731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tatatacgac tcactatagg gagagagcta tgacgtcgca tgcacgcgat caaccacttt
       61 gtacaagaaa gctgggttca agcaaagtac atggtgcgca gagtgtcttg gtgaacctgg
      121 accgccttgg ccagtgcttg gtggtctcgc ccgttagcct gcccagcggt atgggctcct
      181 tcagcagcgt catgttcctt atccaccagg tggtacctgg cccggaaggc caccaggtga
      241 gcgtagtatg ctggcgctgg gatggacacg gagcgtgtgc agcgcacgta ggtgtgacac
      301 agctggtagg ttaggatctg cagctcatca gaggagaaac gattgtcgtc ccagaggacg
      361 tgatagtgcg aaggcctgct tgtcccctgg atgccagcgt gactacacag gtagaagtcg
      421 aactcggtgg ggtgggtgat tttcgtgtcc acagtcgtgc ctgctggaat gtttccactt
      481 ttcccaaccc gctcgttctt gtcagtgcag aagagccggg tgtggtgcct cttctgcacc
      541 acgatgaagg tgatccctgg ctggtagtct ttttctagct tgatacaggc ctcacggatg
      601 gccagcaact cgtggtggag aacctgctgg aactggcctt cagagacacc gtcgcggtag
      661 aagatgatgc gggtgggctt gaagcgcgtg gacttgtaga actggatgag gagctcgcgg
      721 accatggcgg ccaggtcttg tatgatctcc tgccggtgct gctgcacacg cacggtggcg
      781 cagtagcgat tggggtgggc gtccatgctg cccaccacgg cggcaatgga gggcttcttc
      841 ccatccccgg cgggggggtg agtgacgtct gctcccagaa agatgacggg ctgctggaac
      901 accggcggcc tgccctgggg cagcaggatg ttgttcacgc ctcccagctt gacg
//
