LOCUS       sequence-111071-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pDEST26 Ago2 5A
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    143..167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg gactctagcc taggccgcgg accatggcgt actaccatca
      121 ccatcaccat cactctagat caacaagttt gtacaaaaaa gcaggcttaa tgtactcggg
      181 agccggcccc gcacttgcac ctcctgcgcc gccgcccccc atccaaggat atgccttcaa
      241 gcctccacct agacccgact ttgggacctc cgggagaaca atcaaattac aggccaattt
      301 cttcgaaatg gacatcccca aaattgacat ctatcattat gaattggata tcaagccaga
      361 gaagtgcccg aggagagtta acagggaaat cgtggaacac atggtccagc actttaaaac
      421 acagatcttt ggggatcgga agcccgtgtt tgacggcagg aagaatctat acacagccat
      481 gccccttccg attgggaggg acaaggtgga gctggaggtc acgctgccag gagaaggcaa
      541 ggatcgcatc ttcaaggtgt ccatcaagtg ggtgtcctgc gtgagcttgc aggcgttaca
      601 cgatgcactt tcagggcggc tgcccagcgt cccttttgag acgatccagg ccctggacgt
      661 ggtcatgagg cacttgccat ccatgaggta cacccccgtg ggccgctcct tcttcaccgc
      721 gtccgaaggc tgctctaacc ctcttggcgg gggccgagaa gtgtggtttg gcttccatca
      781 gtccgtccgg ccttctctct ggaaaatgat gctgaatatt gatgtgtcag caacagcgtt
      841 ttacaaggca cagccagtaa tcgagtttgt ttgtgaagtt ttggatttta aaagtattga
      901 agaacaacaa aaacctctga cagattccca aagggtaaag tttac
//
