LOCUS       sequence-111073-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pDEST26 Ago2 5A
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      718..727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacgactcac tatagggaga gagctatgac gtcgcatgca cgcgatcaac cactttgtac
       61 aagaaagctg ggttcaagca aagtacatgg tgcgcagagt gtcttggtga acctggaccg
      121 ccttggccag tgcttggtgg tctcgcccgt tagcctgccc agcggtatgg gctccttcag
      181 cagcgtcatg ttccttatcc accaggtggt acctggcccg gaaggccacc aggtgagcgt
      241 agtatgctgg cgctgggatg gacacggagc gtgtgcagcg cacgtaggtg tgacacagct
      301 ggtaggttag gatctgcagc tcatcagagg agaaacgatt gtcgtcccag aggacgtgat
      361 agtgcgaagg cctgcttgtc ccctggatgc cagcgtgact acacaggtag aagtcgaact
      421 ctgtggggtg ggtgattttc gtgtccacag tcgtgcctgc tggaatgttt ccacttttcc
      481 caacccgctc gttcttgtca gtgcagaaga gccgggtgtg gtgcctcttc tgcaccacga
      541 tgaaggtgat ccctggctgg tagtcttttt ctagcttgat acaggcctca cggatggcca
      601 gcaactcgtg gtggagaacc tgctggaact ggccttcaga gacaccgtcg cggtagaaga
      661 tgatgcgggt gggcttgaag cgcgtggact tgtagaactg gatgaggagc tcgcggacca
      721 tggcggccag gtcttgtatg atctcctgcc ggtgctgctg cacacgcacg gtggcgcagt
      781 agcgattggg gtgggcgtcc atgctgccca ccacggcggc aatggagggc ttcttcccat
      841 ccccggcggg ggggtgagtg acgtctgctc ccagaaagat gacgggctgc tggaacaccg
      901 gcggcctgcc ctggggcagc aggatgttgt tcacgcctcc cagcttgacg ttgatcttca
      961 gg
//
