LOCUS       sequence-111074-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Caspase-3 (C163A)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caagtgataa
      121 aaatagagtt cttttgtgag catggaaaca atacatggaa tctgtttctt tgcatgaaaa
      181 gtagcgtcaa aggaaaagga ctcaaattct gttgccacct ttcggttaac ccgggtaaga
      241 atgtgcataa attcaagctt gtcggcatac tgtttcagca tggcacaaag cgactggatg
      301 aaccaggagc catcctttga atttcgccaa gaataataac caggtgctgt ggagtatgca
      361 tacaagaagt cggcctccac tggtatttta tgacacgcca tgtcatcatc aacaccactg
      421 tctgtctcaa tgccacagtc cagttctgta ccccgggcgg cctgaataat gaaaagtttg
      481 ggttttccag ttagacttct acaacgatcc cctctgaaaa agtttgttat ttttttcagg
      541 tcaacaggtc catttgttcc aaaaattatt ccttcttcac catggctcag aagcacacaa
      601 acaaaactgc tccttttgct gtgatcttct ttagaaacat cacgcatcaa ttccacaatt
      661 tcttcacgtg taagatcatt tttattcctg acttcata
//
