LOCUS       sequence-111075-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: ERalpha (Y537S mutant)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaat cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaagac
      121 cgtggcaggg aaaccctctg cctcccccgt gatgtaatac ttttgcaagg aatgcgatga
      181 agtagagccc gcagtggcca agtggctttg gtccgtctcc tccacggatg cccctccacg
      241 gctagtgggc gcatgtaggc ggtgggcgtc cagcatctcc agcagcagat ctgagagggg
      301 caccacgttc ttgcacttca tgctgtacag atgctccatg cctttgttac tcatgtgcct
      361 gatgtgggag aggatgagga ggagctgggc cagccgctgg tgctgctgct gcagggtcag
      421 gcctgccttg gccatcaggt ggatcaaagt gtctgtgatc ttgtccagga ctcggtggat
      481 atggtccttc tcttccagag acttcagggt gctggacaga aatgtgtaca ctccagaatt
      541 aagcaaaata atagatttga ggcacacaaa ctcctctccc tgcagattca tcatgcggaa
      601 ccgagatgat gtagccagca gcatgtcgaa gatctccacc atgccctcta cacattttcc
      661 ctggttcctg tccaagagca agttaggagc aaacagtagc
//
