LOCUS       sequence-111081-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pcDNA 3.1 (+) FLAG-Ago2 S387A
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..139
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag cgtttaaact taagcttggt accgagctca ccatggacta
      121 caaagacgat gacgacaagg gatccgaatt cggtgttctc tctgccattc ccgcacttgc
      181 acctcctgcg ccgccgcccc ccatccaagg atatgccttc aagcctccac ctagacccga
      241 ctttgggacc tccgggagaa caatcaaatt acaggccaat ttcttcgaaa tggacatccc
      301 caaaattgac atctatcatt atgaattgga tatcaagcca gagaagtgcc cgaggagagt
      361 taacagggaa atcgtggaac acatggtcca gcactttaaa acacagatct ttggggatcg
      421 gaagcccgtg tttgacggca ggaagaatct atacacagcc atgccccttc cgattgggag
      481 ggacaaggtg gagctggagg tcacgctgcc aggagaaggc aaggatcgca tcttcaaggt
      541 gtccatcaag tgggtgtcct gcgtgagctt gcaggcgtta cacgatgcac tttcagggcg
      601 gctgcccagc gtcccttttg agacgatcca ggccctggac gtggtcatga ggcacttgcc
      661 atccatgagg tacacccccg tgggccgctc cttcttcacc gcgtccgaag gctgctctaa
      721 ccctcttggc gggggccgag aagtgtggtt tggcttccat cagtccgtcc ggccttctct
      781 ctggaaaatg atgctgaata ttgatgtgtc agcaacagcg ttttacaagg cacagccagt
      841 aatcgagttt gtttgtgaag ttttggattt taaaagtatt gaagaacaac aaaaacctct
      901 gacagattcc caaagggtaa agtttaccaa agaaattaaa ggtctaaagg tggagataac
      961 gcactgtggg cagatgaaga g
//
