LOCUS       sequence-111082-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pcDNA 3.1 (+) FLAG-Ago2 S387A
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      663..672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagactcga gcggccgctc aagcaaagta catggtgcgc agagtgtctt ggtgaacctg
       61 gaccgccttg gccagtgctt ggtggtctcg cccgttactc tgcccagagg tatggcttcc
      121 ttcagcactg tcatgttcct tatccaccag gtggtacctg gcccggaagg ccaccaggtg
      181 agcgtagtat gctggcgctg ggatggacac ggagcgtgtg cagcgcacgt aggtgtgaca
      241 cagctggtag gttaggatct gcagctcatc agaggagaaa cgattgtcgt cccagaggac
      301 gtgatagtgc gaaggcctgc ttgtcccctg gatgccagcg tgactacaca ggtagaagtc
      361 gaactcggtg gggtgggtga ttttcgtgtc cacagtcgtg cctgctggaa tgtttccact
      421 tttcccaacc cgctcgttct tgtcagtgca gaagagccgg gtgtggtgcc tcttctgcac
      481 cacgatgaag gtgatcccgg gctggtagtc tttttctagc ttgatacagg cctcacggat
      541 ggccagcaac tcgtggtgga gaacctgctg gaactggcct tcagagacac cgtcgcggta
      601 gaagatgatg cgggtgggct tgaagcgcgt ggacttgtag aactggatga ggagctcgcg
      661 gaccatggcg gccaggtctt gtatgatctc ctgccggtgc tgctgcacgc gcacggtggc
      721 gcagtagcga ttggggtggg cgtccatgct gcccaccacg gcggcaatgg agggcttctt
      781 cccatccccg gcgggggggt gagtgacgtc tgctcccaga aagatgacgg gctgctggaa
      841 caccggcggc ctgccctggg gcagcaggat gttgttcacg cctcccagct tgacgttgat
      901 cttcaggcag aggttggaca gggtctgtgg cgtggtcctc tgcacgttct tcatctgcac
      961 gcactgcgtg gccatcccca gcaccgtgtc tcccacgcgc ttgacctcgg cgtacacggg
//
