LOCUS       sequence-111108-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-SSFV-dCas9-GFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..776)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(789..809)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaggttgat tatctagatt accctactcg aggctcccat cgatgccaga gcggccgcca
       61 gatttgtaca gttcatccat accatgcgtg atgcccgctg cggttacgaa ctccagcaga
      121 accatatgat cgcgtttctc gttcggatct ttagacagaa cgctttgcgt gctcagatag
      181 tgattgtctg gcagcagaac aggaccatca ccgattggag tgttttgctg gtagtgatca
      241 gccagctgca cgctgccatc ctccacgttg tggcgaattt taaaattcgc tttaatgcca
      301 tttttttgtt tatcggcggt gatgtaaaca ttgtggctgt taaaattgta ttccagctta
      361 tggcccagga tattgccgtc ttctttaaag tcaatgcctt tcagctcaat gcggtttacc
      421 agggtatcgc cttcaaattt cacttccgca cgcgttttgt acgtgccgtc atccttaaag
      481 gaaatcgtgc gttcctgcac atagccttcc ggcatggcgg acttgaagaa gtcatgctgc
      541 ttcatatggt ccggataacg agcaaagcac tgaacaccat aagtcagcgt cgttaccaga
      601 gtcggccaag gtaccggcag tttaccagta gtacagatga acttcagcgt cagtttacca
      661 ttagttgcgt caccttcacc ctcgccacgc acggaaaact tatgaccgtt gacatcacca
      721 tccagttcca ccagaatagg gacgacacca gtgaacagct cttcgccttt acgcatactt
      781 ccggatccta ccttcctctt cttcttggga gagccactag tagagccgtc tccaccgagc
      841 tgagagaggt cgattcttgt ttcatagagc c
//
